AdvanceBio Gly-X 2-AB Express labeling module, 96-ct

SKU: GX96-401 Categories: , Tag:

Description

  • Utilizes 2-AB (2-aminobenzamide) dye to derivatize released glycans.
  • The proven 2-AB dye assures data continuity for ongoing projects.

Specifications

  • Application : Small molecule size for improved chromatographic peak resolution
  • Count : 96
  • Kit Configuration : Sample Prep Kit
  • Recovery : 80 %
  • Precision (well-to-well, day-to-day) : 5% CV
  • Protein Load Range : 1-40 µg
  • Technique : LC & LC/MS
  • Label : 2-AB
  • Deglycosylation Efficiency : 99 %
  • Stability : At least 1 month at -20°C, 5 days at 4–8°C
  • Prep Time : 130 min (24 samples)
  • Equipment and Reagents Provided by User
    • Two dry block heaters with 96 well PCR heat blocks. One of the heaters can be substituted with a 96-well thermocycler. Denaturation and deglycosylation can be performed using a thermocycler. A heat block is required for the labeling step.
    • Two Thermo Scientific 88871001 Compact dry block heaters with 96 well PCR heat blocks.
    • Gly-X vacuum manifold spacer and heater block lid (included in GX400 Gly-X 2-AB Express starter kit, or use GX100 spacer and alternate heater lid)
    • Vacuum manifold (Millipore MSVMHTS00)
    • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L Pump 2522)
    • DI water, ~100 µl per sample
    • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended), ~4 mL per sample
  • Preservation of Sialylated Species : 99%

https://www.agilent.com/store/productDetail.jsp?catalogId=GX96-401

* Available in 24-ct kit, GX24-401 https://www.agilent.com/store/productDetail.jsp?catalogId=GX24-401

AdvanceBio Gly-X 2-AB Express labeling module, 96-ct
$1.00