Description
Hypersil GOLD HPLC columns are available in 12 different chemistries to optimize separations and maximize productivity. The extensive range of Hypersil GOLD columns offers chromatographers outstanding peak shape for reversed phase, ion exchange, HILIC or normal phase chromatography.
With all 12 phases being available with 1.9 μm particle size, Hypersil GOLD columns offer chromatographers flexibility in choosing the correct column, whether they are using conventional or ultra-high pressure LC systems.
These general-purpose columns for HPLC and LC-MS applications provide exceptional peak shape affording confidence in your analytical data. With minimal peak tailing, these columns are packed to achieve exceptional efficiency, peak shape and resolution. Thermo Scientific™ Hypersil GOLD™ columns offer a broad range of phases and particle sizes (1.9, 3, 5 µm). They are your go-to column for starting method development.
| Selectivity | Functionality | Description |
| Reversed-phase hydrophobic interaction of nonpolar compounds (reversed-phase) | C18 | Excellent peak shape for hydrophobic interaction |
| C8 | Similar selectivity to C18 but with less retention | |
| C4 | Short alkyl chain length, low hydrophobicity column for less retention than C18 or C8 | |
| Reversed-phase with enhanced hydrolytic stability | aQ | Polar endcapped C18 are stable in 100% aqueous mobile phases and provide enhanced retention and resolution of polar analytes |
| Reversed-phase with selectivity for aromatic compounds | PFP: | Alternative selectivity in reverse phase applications. The fluorine atoms around the phenyl ring enhance pi-pi interactions with aromatic molecules, including hologenated compounds |
| pentafluoro- phenyl | ||
| Phenyl | Alternative selectivity in reverse phase applications, particularly for aromatic compounds | |
| Normal phase / reversed-phase | CN: cyano | For both reversed and normal phase separations; alternative selectivity with lower hydrophobidity. |
| Multi Mode: WAX / RP / NP | Amino | Can be used in four modes: reversed-phase, normal phase, ion exchange and HILIC; especially good for carbohydrates |
| Anion exchange | AX: anion exchange | Separate proteins, peptides, anionic species and polar molecules |
| SAX: strong anion exchange | Highly stable silica-based quarternary amine strong anion exchange column, designed for separating small polar organic analytes in aqueous mobile phase | |
| Normal phase of non- polar and moderately polar organic compounds | Silica | Unbonded silica |
| HILIC; enhanced retention of polar and hydrophilic compounds | HILIC | Polyethyleneimine functional group |
