MP Biomedical FastPrep DNA & RNA Isolation Kits, Protein Extraction and Purification

Description

DNA & RNA Isolation Kits and Reagents

A comprehensive range of solutions for the isolation and purification of DNA and RNA from any source.

Isolation and purification of nucleic acids is at the heart of every molecular biology laboratory and must be performed routinely, with the highest yield and efficiency. We offer a range of nucleic acid extraction kits and reagents designed to reliably and robustly generate high yields of high-quality genomic DNA, plasmid DNA, RNA or total nucleic acid from a variety of sample types.

Genomic DNA Isolation

A wide variety of specialized kits for manual and automated isolation of genomic DNA

Plasmid DNA Purification

Miniprep kits for high-quality plasmid DNA purification

DNA Clean-up

Comprehensive range of kits for the purification of DNA from agarose gels, PCR and other enzymatic reactions

Total RNA Isolation

Manual and automated total RNA isolation kits from a wide range of sample types

RNA Clean-up

Complete kits for the purification of RNA from agarose or polyacrylamide gels and other solutions

Individual reagents for DNA and RNA Isolation

Portfolio of reagents used for the isolation and purification of DNA and RNA

Protein Extraction and Purification

Challenges in Nucleic Acid Extraction

Metagenomic studies involve isolation of nucleic acids from the entire biome of a given sample. Environmental or gut samples can present significant challenges in terms of sample preparation and subsequent isolation and purification.

Typical soil, sludge, and fecal samples exhibit variables that can make processing procedures difficult to standardize. These variables include: complex matrices with varying mechanical and rheological properties; diverse biological materials including microorganisms, plant and animal tissue, and other cells; and innate PCR inhibitors and degrading enzymes.

Cited in over 8000 scientific publications, FastDNA Spin Kits for Soil and FastRNA Pro Soil Kits deliver the highest yields of contaminant-free DNA and RNA from these samples. They contain Lysing Matrix tubes and all the reagents needed to extract DNA and RNA in a process that efficiently removes humic acids and other PCR inhibitors.

RNA Isolation and Purification Kits

Isolate Genomic DNA from Plants, Bacteria, Algae, Fungi, Soil or Feces

High performance FastDNA™ purification kits including Lysing Matrix tubes provide ready-to-use methods for the release and subsequent purification of intact DNA from virtually any source. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired  application.

FastDNA™ SPIN Kit

Rapid Method of Isolating Pure Genomic DNA from a Wide Variety of Sources! Isolate PCR-ready DNA from a variety of sample types. No hazardous organic reagents are required. The FastDNA™ SPIN Kit quickly and efficiently isolates genomic DNA from almost any sample (plant and animal tissues, cultured cells, bacteria, yeast, fungi, insects, etc). Up to 200 mg of tissue or cells are processed by the FastPrep-24™ or FastPrep® FP120 with the Lysing Matrix A tubes. The kit includes 3 different chaotropic buffers for the homogenization of a wide variety of sample types and the released DNA is purified by a silica-based spin filter method. Purified DNA is ready for enzyme digestion,
electrophoresis, PCR and any other desired application.

FastDNA™ SPIN Kit For Soil

Isolate Pure DNA from Cells Present in Soil or Other Environmental Samples! Easily isolate DNA from a variety of organisms in many different types of soil. No hazardous organic reagents are required The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep®-24 or FastPrep® FP120 with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR and other downstream applications.

FastDNA™ SPIN Kit for Feces

Isolate High Quality DNA from Fecal Material! Removes organic contaminates, such as humic acid, for downstream applications achieves Optimal 260/280 ratios (1.8-2.0). Typically isolates 10 μg – 20 μg of gDNA from 500 mg of stool FastDNA™ SPIN Kit designed exclusively for the isolation of genomic DNA from fecal material. With the FastDNA™ SPIN Kit for Feces you will have everything you need to quickly and efficiently lyse any fecal sample isolating high quality DNA for immediate use in downstream applications. Used in conjunction with our FastPrep-24™ homogenization system, you will be able to completely lyse fecal samples in seconds with no pre-grinding or preparation.

FastDNA™ Kit

Rapid Method of Isolating Pure Genomic DNA from a Wide Variety of Sources. Isolate PCR-ready DNA from a variety of sample types. No hazardous organic reagents are required. The FastDNA™ Kit quickly and efficiently isolates high quality genomic DNA from plants, animals, bacteria, yeast, algae, and fungi using Lysing Matrix A (garnet and one 1/4 inch ceramic bead) for cell lysis and a silica-based method for the
purification process. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.

FastDNA™ Spin Kit for Plant and Animal Tissue

Isolate High Quality DNA from Plant and Animal Tissue! Rapid and reproducible sample lysis with any FastPrep Instrument. Achieves Optimal 260/280 ratios (1.8-2.0). The FastDNA™ Spin Kit for Plant and Animals Tissues quickly and efficiently isolates high quality genomic DNA from plant and animal tissues using Lysing Matrix D (1.4mm ceramic beads) for cell lysis and a silica-based spin filter method
for the purification process.

RNA Isolation and Purification Kits

Isolate Total RNA From Plants, Animals,Bacteria, Yeast, Algae, and Fungi

FastRNA™ SPIN Kits quickly and efficiently isolates high-quality, total RNA from bacterial cell culture, yeast strains, fungi and algae in approximately 15 minutes using a specialized Lysing Matrix for cell lysis and SPIN columns for the purification process.
FastRNA™ Pro Kits use the traditional RNApro™ Solution to efficiently inactivate cellular RNases during cell lysis, thus preventing RNA degradation. The RNA is purified and isolated by chloroform extraction and ethanol precipitation. Both kit formats utilize FastPrep® homogenization, and the purified RNA is ready for downstream applications, including RT-PCR and northern analysis.

FastRNA™ Spin Kit for Microbes

The FastRNA™ SPIN Kit for Microbes quickly and efficiently isolates high-quality, total RNA from tough-to-lyse bacterial cell cultures and other microbial sources in approximately 15 minutes using a zirconium silicate Lysing Matrix (0.1mm) to lyse bacteria and SPIN columns for the purification process.

FastRNA™ Spin Kit for Yeast

The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialized Lysing Matrix (Yttria-stabilized zirconium oxide, 0.5mm) for cell lysis and SPIN columns for the purification process.

RNA Isolation and Purification Pro Kits

The FastRNA™ Pro Soil-Direct and Indirect kits are designed to efficiently isolate total RNA from organic material found in soil samples or soil supernatants. The direct method consists of extracting nucleic acid from microorganisms and other biological specimens directly from soil. The indirect method utilizes an initial separation of microorganisms and other biological specimens from the soil followed by lysis of the organisms and RNA purification. This method also permits soil incubation with growth media in order to amplify under-represented living organisms prior to RNA isolation if specific comparisons of microbial diversity are not desired. FastRNA™ Pro Soil kits purify RNA in a process that removes humic substances and other inhibitors, and efficiently inactivates cellular RNases during homogenization to prevent RNA degradation. Purified RNA is thus suitable for RT-PCR analysis and many other downstream applications.

FastRNA™ Pro Soil Kits

Isolate Total RNA from Soil! Easily lyse difficult gram positive cells, plant material, and organic debris directly from soil. Lysis and purification solutions protect RNA during processing. Humic acids levels reduced to allow uninhibited RT-PCR.

FastRNA™ Pro Soil-Direct Kit

The FastRNA™ Pro Soil-Direct Kit is designed to efficiently isolate total RNA from organic material found in soil samples. Using any FastPrep instrument, up to 0.5g of material is homogenized by Lysing Matrix E impact-resistant 2ml tubes. Total RNA is released into a protective solution, extracted, precipitated and cleaned with the proprietary RNAMATRIX and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR and other downstream applications.

FastRNA™ Pro Soil-Indirect Kit

The FastRNA™ Pro Soil-Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil supernatants. Cellular material is washed away from soil particles, centrifuged, and homogenized by any FastPrep® Instrument in impact-resistant 2 mL tubes of Lysing Matrix E. Total RNA is released into a protective solution, extracted, precipitated, and cleaned with the proprietary RNAMATRIX™ and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR analysis and other downstream applications.

FastRNA™ Pro Kits

Isolate High Quality Total RNA with a Single-Reagent Extraction Method! Safe and consistent RNA isolation with the single-reagent RNAProTM solution. Lyse and isolate total RNA from a variety of sample types. The FastRNA™ Pro Kits are designed to quickly and efficiently isolate total RNA from samples. During the homogenization step, intact total RNA is released in the proprietary RNAPro™ solution where it is immediately stabilized. The RNAPro™ solution inactivates cellular RNases during cell lysis to prevent RNA degradation. RNA is then extracted with chloroform and precipitated with ethanol. DEPC-treated water is provided for re-suspension of total RNA. High
quality RNA prepared with FastRNA™ Pro Kits is ready for all downstream applications including RT-PCR, gene expression and microarray analysis.

FastRNA™ Pro Blue Kit (Bacteria)

The FastRNA® Pro Blue Kit is designed to efficiently isolate total RNA from gram positive and gram negative bacteria. Using any FastPrep Instrument, up to 1010 cells are homogenized by Lysing Matrix B in impact-resistant 2 mL tubes. Total RNA is released into the proprietary, protective RNApro™ Solution. Following extraction with chloroform and precipitation, total RNA is extracted.

FastRNA™ Pro Red Kit (Yeast and Fungi)

The FastRNA™ Pro Red Kit is designed to efficiently isolate total RNA from yeast and fungi. Using any FastPrep® Instrument, up to 109 cells are homogenized by Lysing Matrix C in impact-resistant 2 mL tubes. Total RNA is released into the proprietary, protective RNApro™ Solution. Following extraction with chloroform and precipitation, total RNA is extracted.

FastRNA™ Pro Green Kit (Plant and Animal)

The FastRNA™ Pro Green Kit is designed to efficiently isolate total RNA from any type of plant and animal tissue or cultured cells. Using any FastPrep® Instrument, between 50-500 mg of tissue can be homogenized by Lysing Matrix D in impact-resistant 2 mL tubes. Total RNA is released into the proprietary, protective RNApro™ Solution. Following extraction with chloroform and precipitation, total RNA is extracted.

 

Protein Isolation and Purification Kits

The FastPROTEIN™ products employ a powerful, patented technology for the rapid lysis of yeast and bacteria. Used in conjunction with any FastPrep® Instrument, these products offer the fastest way to release expressed proteins from the host organism. FastPROTEIN™ Kits are perfect for analyzing protein expression conditions using gel analysis. Samples are enclosed during the quick lysis step, thus preventing cross-contamination or sample loss. Save valuable time by reducing sample lysis time to seconds! Total proteins isolated with the FastProtein™ Matrices are native and can be used for a variety of applications including SDS-PAGE, Western Blotting, immunoprecipitation, gel mobility shift assays, and enzyme activity analysis.

FastProtein™ Blue Matrix

The FastPROTEIN™ Blue matrix is optimal for lysing gram positive and gram negative bacteria. These fine glass beads are designed for use with gram positive bacteria or any difficult microorganism. Cells, resuspended in either 1X PBS or your own expression buffer, are added to the Lysing Matrix and processed in any FastPrep® Instrument for 20-40 seconds.

FastProtein™ Red Matrix

The FastPROTEIN™ Red matrix is used to lyse yeast cells. Cells, re-suspended in either Yeast Breaking Buffer (YBB-supplied with the kit) or your own expression buffer, are added to the small glass beads of this Lysing Matrix and processed in any FastPrep® Instrument for 20-40 seconds.

FastGlycoProtein™ Isolation Kit ConA Resin

An optimized Lysing Matrix A, coupled with any FastPrep® Instrument, quickly lyses most tissue samples within 40 seconds. Following lysis, samples are loaded to the ConA lectin resin SPIN filter tubes. The resin is washed and the Glycoproteins are eluted with the elution buffer. Eluted Glycoproteins are ready for 1-D gel electrophoresis and total protein (Bradford type) Assays. Other downstream applications may require sample processing to remove incompatible substances in the elution buffer.

FastGlycoProtein™ Isolation Kit WGA Resin

An optimized Lysing Matrix A, coupled with any FastPrep® Instrument, quickly lyses most tissue samples in 40 seconds or less. Following lysis, samples are loaded to the WGA lectin resin SPIN filter tubes. The resin is washed and the Glycoproteins are eluted with the elution buffer. Eluted Glycoproteins are ready for 1-D gel electrophoresis and total protein (Bradford type) Assays. Other downstream applications may require sample processing to remove incompatible substances in the elution buffer.