Glycan Hydrazinolysis Kit

$1.00

SKU: GK50202 Categories: , Tag:

Description

  • Nonselective release and recovery of intact glycans from glycoproteins using hydrazinolysis.
  • Up to 18 samples may be processed (3 batches of 6).

Specifications

  • Count : 18
  • Applications :
    • Cleavage independent of the glycan structure or the primary sequence of the glycoprotein.
    • Glycan recovery typically 90%. Released glycans contain a free reducing end.
    • Cleavage differentiation between N- and O-linked glycans.
    • Sample size: 50 – 500 µg. Up to 18 individual samples (3 batches of 6).
  • Equipment and Reagents Provided by User
    • Glass syringe, gas-tight with Teflon plunger
    • Lyophilizer
    • Desiccator with suitable drying agent
    • Heating block (for 12-mm vials), sand bath or dry oven set to 60°C or 95°C
    • CAP Type I water supply (Milli-Q or equivalent)
    • Dialysis membrane (5 – 10 kDa cutoff)
    • SpeedVac evaporator or equivalent with a rotor for 12-mm (outer diameter) vials or vacuum evaporator with charcoal/alumina trap.
    • Vortex mixer
    • Dry nitrogen or argon (optional)

https://www.chem.agilent.com/store/productDetail.jsp?catalogId=GK50202&catId=SubCat2ECS_654386

Glycan Hydrazinolysis Kit
$1.00