DMB Sialic Acid Labeling Kit
Description
DMB Sialic Acid Labeling Kit (formerly ProZyme)
- Release and derivatization of sialic acids with 1,2-diamino4,5-methylenedioxybenzene (DMB) for separation by liquid chromatography.
- Comparison may be made against the included sialic acid reference panel (SARP, p/n GKRP-2503), which includes:
- Neu5Gc (NGNA)
- Neu5Ac (NANA)
- Neu5,7Ac2
- Neu5Gc,9Ac
- Neu5,9Ac2
- Neu5,7(8)9Ac3.
- Up to 20 samples may be processed (2 batches of 10).
Count : 10
Protein Load Range : 1-10 µg
Equipment and Reagents Provided by User :
- Heating block or oven (set at 50EC for labeling, 80EC for release reaction)
- HPLC system for analysis
- GlykoSep R HPLC column
- Water, HPLC grade
- Methanol, HPLC grade
- Acetonitrile, HPLC grade
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