Description
Enzyme Controls
Analysis of cytochrome P450 form specific metabolism requires use of the appropriate control preparation in order to eliminate the possibility of metabolism by enzymes native to the cell lines. Corning Life Sciences prepares control microsomes and cytosols using procedures identical to those used to prepare other microsomal products.
For P450 enzymes, control microsomes are available from both Sf9 and High Five (Hi5 or BTI-TN-5B1-4) insect cell preparations. All insect cell control microsomes are prepared from insect cells infected with wild-type baculovirus. Control preparations are also available for UGT, MAO, NAT, and CES Corning® Supersomes™ enzymes products.
Normalization of Protein Concentration
Differences in total protein concentration can affect the free concentration of substrate and hence enzyme kinetics. The available substrate concentration may be significantly lower than initially assumed for those substrates which have a large potential to bind nonspecifically to protein (J. Pharmacol. Exp. Ther. 283:46 [1997]; Drug Metab. Dispos. 25:1359 [1997]). Total protein concentration can be controlled and normalized in each incubation by the addition of control microsomes.
| Cat. No. | Product Name |
| 456200 | Corning® Supersomes™ Insect Cell Microsomes, Negative Control, 1.5 mL |
| 456201 | Corning® Supersomes™ Insect Cell Microsomes, Negative Control, 0.5 mL |
| 456244 | Corning® Supersomes™ Human P450 OxidoReductase + b5, Negative Control, 0.5 mL |
| 456280 | Corning® Supersomes™ Negative Control for Monoamine Oxidase (MAO), 0.5 mL |
| 456299 | Corning® Supersomes™ Sf9 Insect Cell Control, 0.5 mL |
| 456400 | Corning® Supersomes™ Insect Cell Control Microsomes for UGTs, 0.5 mL |
| 456800 | Corning® Supersomes™ Ultra Cytosol Negative Control, 5 mg/mL, 0.5 mL |
