AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL

SKU: GKE-5016A Categories: ,


  • A recombinant form of PNGase F (100 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn.
  • Includes
    • 5x N-glycanase reaction buffer,
    • denaturation solution,
    • detergent solution,
    • 5x N-glycanase Tris reaction buffer (MS applications).


  • Enzyme Specific Activity : ≥10 units/mg
  • Volume : 40 µL
  • Application : Glycoprotein deglycosylation
  • pH Range : 7.5-9.5
  • Enzyme Specificity
    • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans.
    • Asparagine must be peptide bonded at both termini.
    • Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage.
    • This product is Endo F free.
  • Enzyme Applications
    • Release of intact N-linked glycans from glycopeptides and glycoproteins.
    • Structure-function studies of N-glycosylated glycoproteins.
    • Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies
  • Enzyme Unit Definition : One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
  • Enzyme Formulation : 20 mM Tris-HCl, 50 mM NaCl, (pH 7.5)
  • Enzyme Source :
    • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli.
    • The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum.
    • Enzyme is also known asPNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
  • Concentration : 2.5 U/mL
  • Unit : 100 mU
  • Molecular Weight : ~35,000 daltons
  • pH Optimum : 8.6

*Available in different unit and packing size

GKE-5016A, 100mU

GKE-5016A-05, 5x 100MU

GKE-5016B, 200mU

GKE-5016B-1, 5x 200mU

GKE-5006D, 1U

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL