AdvanceBio Gly-X N-glycan prep with InstantQ kit, 96-ct

$1.00

SKU: GX96-IQ Categories: , Tag:

Description

  • Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye.
  • After a simple cleanup step, the glycan samples are ready for analysis on the Gly-Q glycan analysis system.
  • With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation.
  • Includes
    • GX96-300 Gly-X deglycosylation module for InstantQ,
    • GX96-301 Gly-X InstantQ labeling module,
    • GX96-302 Gly-X InstantQ cleanup module,
    • GKSQ-505 Gly-Q alignment standards set (GKSQ-500 & GKSQ-503),
    • GQ103 Gly-Q cartridge module.

Specifications

  • Instrument Compatibility : Gly-Q Glycan Analysis System
  • Count : 96
  • Protein Load Range : 1-40 µg
  • Technique : CE
  • Stability : up to 18 hrs on RT autosampler, and -20°C for up to 30 days in water
  • Prep time (approx) : ~1 hour
  • Preservation of Sialylated Species : 95 %
  • Application :
    • Flexible, high-throughput format: process 1 to 96 samples
  • Preservation of Sialylated Species : 95 %
  • Kit Configuration : Sample Prep Kit
  • Precision (well-to-well, day-to-day) : 5% CV
  • Recovery : 85 %
  • Label : InstantQ
  • Deglycosylation Efficiency : 99 %
  • Equipment and Reagents Provided by User
    • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
    • Thermocycler (Corning THERM-1001, 110V. THERM-1000, 230V)
    • Vacuum manifold (Millipore MSVMHTS00-HTS)
    • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L pump 2522)
    • Formic acid, MS-grade (Fisher A117-50 recommended)
    • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)

https://www.agilent.com/store/productDetail.jsp?catalogId=GX96-IQ

*Available in 24-ct format, GX24-IQ https://www.agilent.com/store/productDetail.jsp?catalogId=GX24-IQ

AdvanceBio Gly-X N-glycan prep with InstantQ kit, 96-ct
$1.00