Description
- Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye.
- After a simple cleanup step, the glycan samples are ready for analysis on the Gly-Q glycan analysis system.
- With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation.
- Includes
- GX96-300 Gly-X deglycosylation module for InstantQ,
- GX96-301 Gly-X InstantQ labeling module,
- GX96-302 Gly-X InstantQ cleanup module,
- GKSQ-505 Gly-Q alignment standards set (GKSQ-500 & GKSQ-503),
- GQ103 Gly-Q cartridge module.
Specifications
- Instrument Compatibility : Gly-Q Glycan Analysis System
- Count : 96
- Protein Load Range : 1-40 µg
- Technique : CE
- Stability : up to 18 hrs on RT autosampler, and -20°C for up to 30 days in water
- Prep time (approx) : ~1 hour
- Preservation of Sialylated Species : 95 %
- Application :
- Flexible, high-throughput format: process 1 to 96 samples
- Preservation of Sialylated Species : 95 %
- Kit Configuration : Sample Prep Kit
- Precision (well-to-well, day-to-day) : 5% CV
- Recovery : 85 %
- Label : InstantQ
- Deglycosylation Efficiency : 99 %
- Equipment and Reagents Provided by User
- 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
- Thermocycler (Corning THERM-1001, 110V. THERM-1000, 230V)
- Vacuum manifold (Millipore MSVMHTS00-HTS)
- Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L pump 2522)
- Formic acid, MS-grade (Fisher A117-50 recommended)
- Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
https://www.agilent.com/store/productDetail.jsp?catalogId=GX96-IQ
*Available in 24-ct format, GX24-IQ https://www.agilent.com/store/productDetail.jsp?catalogId=GX24-IQ
