AdvanceBio Gly-X N-glycan prep with InstantPC kit, 96-ct

$1.00

Description

Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye.
After a simple cleanup step, the glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods.
With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation.
The InstantPC dye delivers unmatched fluorescent brightness and MS signal, that enables a single labeling method to be deployed across different glycan analysis workflows.
Sample preparation time: ~1 hour.
Includes
- GX96-100 Gly-X deglycosylation module,
- GX96-101 Gly-X InstantPC labeling module,
- GX96-102 Gly-X InstantPC cleanup module.

Specifications

Application :
- Flexible, high-throughput format: process 1 to 96 samples
Kit Configuration : Sample Prep Kit
Count : 96
Recovery : 95 %
Precision (well-to-well, day-to-day) : 5% CV
Protein Load Range : 1-40 µg
Technique : LC & LC/MS
Label : InstantPC
Prep time (approx) : ~1 hour
Stability : Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
Preservation of Sialylated Species : 95 %
Equipment and Reagents Provided by User
- Flexible, high-throughput format: process 1 to 96 samples
- 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V).
- Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
- Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522).
- Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)