AdvanceBio Gly-X N-glycan prep with APTS Express kit, 96-ct

$1.00

SKU: GX96-APTS Categories: , Tag:

Description

  • Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 8-aminopyrene-1,3,6-trisulfonic acid (APTS).
  • With deglycosylation carried out in solution, the method is simple, rapid and suitable for automation.
  • After a simple cleanup step, the APTS-labeled glycan samples are ready for analysis by CE, CE/MS, and other methods.
  • Includes
    • GX96-100 Gly-X deglycosylation module (96-ct),
    • GX96-601 Gly-X APTS Express labeling module (96-ct),
    • GX96-602 Gly-X APTS Express cleanup module (96-ct).

Specifications

  • Application :
    • Flexible, high-throughput format: process 1 to 96 samples
  • Kit Configuration : Sample Prep Kit
  • Count : 96
  • Protein Load Range : 1-40 µg
  • Technique : CE
  • Label : APTS
  • Prep time (approx) : ~2.5 hour
  • Equipment and Reagents Provided by User
    • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C
    • Thermocycler (Corning THERM-1001, 110 V. THERM-1000, 230 V)
    • Vacuum manifold (Millipore MSVMHTS00-HTS)
    • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220V. Welch WOB-L pump 2522)
    • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB/APTS starter kit is included free of charge with first Gly-X order, please contact us for details)
    • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended
    • DI Water

https://www.agilent.com/store/productDetail.jsp?catalogId=GX96-APTS

AdvanceBio Gly-X N-glycan prep with APTS Express kit, 96-ct
$1.00