AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct

$1.00

SKU: GX96-2AB Categories: , Tag:

Description

  • Utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye.
  • With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation.
  • After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods.
  • Includes
    • GX96-100 Gly-X deglycosylation module (96-ct),
    • GX96-401 Gly-X 2-AB Express labeling module (96-ct),
    • GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

Specifications

  • Application :
    • Flexible, high-throughput format: process 1 to 96 samples
  • Count : 96
  • Kit Configuration : Sample Prep Kit
  • Recovery : 80%
  • UNSPSC Code : 41115711
  • Protein Load Range : 1-40 µg
  • Technique : LC & LC/MS
  • Label : 2-AB
  • Deglycosylation Efficiency : 99 %
  • Stability : At least 1 month at -20°C, 5 days at 4–8°C
  • Prep time (approx) : ~2.5 hours
  • Equipment and Reagents Provided by User
    • 96-well Thermocycler or two independent heat blocks
    • Thermocycler (Corning THERM-1001, 110V, THERM-1000, 230V)
    • Vacuum manifold (Millipore MSVMHTS00-HTS)
    • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522)
    • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit – included free of charge with first Gly-X order, please contact us for details)
    • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
    • DI water
  • Preservation of Sialylated Species : 99%

https://www.agilent.com/store/productDetail.jsp?catalogId=GX96-2AB

* Available in 24-ct kit, GX24-2AB https://www.agilent.com/store/productDetail.jsp?catalogId=GX24-2AB

AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct
$1.00