AdvanceBio Gly-X 2-AB Express cleanup module, 96-ct
- Module for use with Gly-X N-glycan prep and labeling with 2-AB Express modules.
- This cleanup plate is used for on-matrix 2-AB labeling as well as cleanup of free dye.
- Application : Optimized cleanup removes excess free dye, protein, and other interfering compounds.
- Count : 96
- Kit Configuration : Sample Prep Kit
- Recovery : 80 %
- Precision (well-to-well, day-to-day) : 5% CV
- Protein Load Range : 1-40 µg
- Technique : LC & LC/MS
- Label : 2-AB
- Deglycosylation Efficiency : 99 %
- Stability : At least 1 month at -20°C, 5 days at 4–8°C
- Prep Time : 130 min (24 samples)
- Equipment and Reagents Provided by User
- Gly-X deglycosylation and 2-AB Express labeling 24-ct [GX24-401AB] or 96-ct [GX96-401AB]
- Vacuum manifold (Millipore MSVMHTS00-HTS)
- Vacuum pump (Millipore WP6211560, 110V. WP6122050, 220V. Welch WOB-L Pump 2522)
- ProZyme vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit, included free of charge with first Gly-X order, please contact us for details)
- Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
- DI water
- Preservation of Sialylated Species : 99