Presentation Abstracts:
Introducing New Exciting Cell Culture Products: Upcell and Hydrocell
We have developed a novel temperature-responsive cell cultureware that allows collection of
cells by mere control of external
temperature, completely abolishing the usage of cell damaging
proteolytic enzymes such as trypsin. The temperature-responsive
polymer (PIPAAm) grafted surface exhibits thermally reversible
hydrophobic-hydrophilic property across the lower critical solution
temperature (LCST) of 32C. At
37C, the surface is slightly hydrophobic, allowing adherent cells to attach. However when the
temperature is reduced to room temperature (20-25C), the surface becomes hydrophilic, prompting attached cells to detach
spontaneously.
This temperature responsive cell cultureware is particularly suitable for preparation of intact single cell suspension for flow
cytometry application by improving the labeling efficiency. Moreover, the cell recovery and viability rate is dramatically improved
for macrophage and dendritic cell culture, which are difficult to detach even if trypsin is used.
When cells are cultured beyond over-confluency, the cells can be collected in a form of thin-film, what so-called as "cell-sheet".
Since trypsin is not utilized, the extra cellular matrix (ECM) deposited by cells are also collected together with cell-sheet. Due to
this intact ECM, the cellsheet does not require any suture when transplanted to the host. Besides, the cell-sheets can be
stacked together to create 3D tissue constructs to mimic "in-vivo" like tissue mass thanks to the intact surface proteins that are
used for cell-cell communication necessary to establish vertical cell-cell gap junction formation..
With this visit, we would like to present our recent findings and application of our novel cell cultureware, UpCell and other
innovative cell culture surface.
5 Solutions to Eradicate Cell Culture Obstacles and Barriers
- Overcoming barriers of cell attachment and detachment
- Maximising productivity of bio-production
- Minimising cell culture contamination
- Safety first’ – Cell preservation
- For your further application – Suspension cell imaging work
Tips for Good Laboratory Pipetting
- Operating principle of pipette
- Pipetting techniques
- What is calibration and adjustment?
- What affects pipetting?
- Why calibrate yourself?
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